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. 1999 Feb;119(2):375–384. doi: 10.1104/pp.119.2.375

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Correlation of mastoparan-induced DNA fragmentation and TE differentiation. Cells cultured either in medium containing inductive levels of hormones (IND) supporting TE differentiation or in medium lacking hormones (NO HORM) (which does not support TE differentiation) were treated with 2.5 μm mastoparan at the indicated times. The percentage of cells exhibiting DNA fragmentation was determined using TUNEL 6 h later. To determine the correlation between the percentage of cells differentiating as TE and the percentage of cells fragmenting DNA in response to mastoparan (inset), cells from two separate cell isolations (♦ and ⋄) were cultured in different media with hormone levels ranging from zero to fully inductive to cause variations in the percentage of cells differentiating as TEs. Cells were treated with 2.5 μm mastoparan at the onset of differentiation and assayed for DNA fragmentation with TUNEL 6 h later. The percentage of TEs formed was determined for both sets of cultures after approximately 96 h of culture.