Table I.
Cell death and DNA fragmentation induced by hydrolytic enzymes
| Hydrolase | Concentration (w/v) | Dead | TUNEL |
|---|---|---|---|
| % | |||
| Control | 25 | 2.7 | |
| Macerozyme | 1 | 40 | 4.8 |
| 0.1 | 27 | 2.5 | |
| Pectinase | 1 | 40 | 0.5 |
| 0.1 | 39 | 3.4 | |
| Cellulase | 1 | 58 | 0 |
| 0.1 | 37 | 1.5 | |
| Proteinase K | 1 | 48 | 0 |
| 0.1 | 17 | 0 | |
| Protease XIV | 1 | 31 | 0 |
| 0.1 | 22 | 1.1 | |
| Protease XXIV | 1 | 54 | 0 |
| 0.1 | 30 | 0 | |
| Protease XVII-B | 1 | 21 | 0 |
| 0.1 | 35 | 0 | |
| Papain | 1 | 61 | 3.2 |
| 0.1 | 19 | 1.1 | |
| Chymotrypsin | 1 | 28 | 0 |
| 0.1 | 22 | 0 | |
| Trypsin | 1 | 96 | 21.9 |
| 0.1 | 55 | 6.0 | |
Cells were treated with the indicated concentration of each hydrolase 67 h after isolation and scored for the percentage of dead cells 6 h later. At least 200 cells were scored for each treatment.