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. 1999 Feb;119(2):385–398. doi: 10.1104/pp.119.2.385

Figure 4.

Figure 4

Separation of neutral and acidic α-glucosidases from an Arabidopsis leaf extract using a ConA-Sepharose column, and the pH profile of each activity peak. A, ConA-Sepharose column. Bound proteins were eluted with 15 mm methyl Glc (MG). α-Glucosidase activity was measured at pH 4.5 (•) and pH 7.0 (○). B, pH profile of pooled fractions 2 and 3 from A. C, pH profile of pooled fractions 12 to 14 from A. For B and C, buffers were sodium acetate (○), Mes (•), or Hepes (▿). U, Unit.