Separation of neutral and acidic α-glucosidases
from an Arabidopsis leaf extract using a ConA-Sepharose column, and the
pH profile of each activity peak. A, ConA-Sepharose column. Bound
proteins were eluted with 15 mm methyl Glc (MG).
α-Glucosidase activity was measured at pH 4.5 (•) and pH 7.0 (○).
B, pH profile of pooled fractions 2 and 3 from A. C, pH profile of
pooled fractions 12 to 14 from A. For B and C, buffers were sodium
acetate (○), Mes (•), or Hepes (▿). U, Unit.