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. 1999 Feb;119(2):385–398. doi: 10.1104/pp.119.2.385

Figure 6.

Figure 6

Separation of the Arabidopsis α-glucosidase activity from most of the 81-kD polypeptide using Sephadex G-150. A, Sephadex G-150 column. α-Glucosidase activity was assayed at pH 4.5 (•) and pH 7.0 (○). Total protein was monitored at A280 (solid line). U, Unit. B, Immunoblot of fractions 5 and 10 from A. Equal volumes (10 μL) of each fraction were separated by SDS-PAGE and probed with anti-Aglu-1.