Table I.
Quantification of immunogold labeling of rice roots with the single-affinity-purified anti-NADH-GOGAT IgG
| Cellular Compartment | Root Tissue Type
|
||
|---|---|---|---|
| Epidermis/Exodermis | Cortex | Central Cylinder | |
| gold particles μm−2 | |||
| Plastid | 155.7 ± 60.21 (13) | 158.0 ± 96.92 (9) | 141.2 ± 37.47 (13) |
| Mitochondrion | 108.8 (2) | N.D.a | 49.94 ± 12.99 (12) |
| Cell wall | 23.94 ± 10.28 (4) | 12.54 ± 3.350 (5) | 12.90 ± 6.569 (6) |
| Cytosol | 9.301 ± 4.810 (4) | 16.16 ± 4.801 (6) | 12.76 ± 5.395 (10) |
| Vacuole | 0.7990 ± 0.5049 (4) | 0.3562 ± 0.0767 (4) | 1.260 ± 0.6878 (6) |
| Air space | N.D. | 0.3148 ± 0.4100 (4) | 10.34 (2) |
Rice seedlings were grown for 24 h with 1 mm NH4+. Six to nine micrographs of each of the three tissue types (epidermis and exodermis, cortex, and central cylinder) were taken from sections of root tip (<10 mm) incubated with single-affinity-purified anti-NADH-GOGAT IgG as the primary antibody, and analyzed as described in Methods. Values represent the mean ± sd immunolabeling density. Numbers in parentheses are n values.
a
N.D., Not detected.