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Relationship between the endogenous ABA level in leaves of M. quadrifolia and the duration that the plants were cultured in MS medium. Leaves from several plants were pooled to give 1 to 2 g fresh weight (gFW) of tissue per sample. Data points represent the averages and sd of samples measured in three independent experiments. The sd in the samples for d 0 and 16 are less than 0.05 nmol/g fresh weight. ABA contents were determined with ELISA using monoclonal antibodies against ABA.
