Table III.
Growth Condition | Leaf Form | ABA Content |
---|---|---|
pmol g−1 fresh wt | ||
Culturea | ||
Control | Submerged | 220.9 ± 11.8 |
Blue Light | Aerial | 250.0 ± 28.5 |
Field | Aerial | 266.4 ± 105.7 |
Aerial leaves grown in the field or induced by blue light in culture contain ABA levels similar to submerged leaves produced in culture. ABA contents are the averages ± sd of three samples. Leaves were pooled from different plants to give 1 to 2 g fresh weight of tissue per sample. ABA contents were detected with ELISA using monoclonal antibodies against ABA.
Plants were cultured in the basal medium (Laetsch, 1967). The leaves were harvested from plants grown in culture for 7 weeks.