. Author manuscript; available in PMC: 2012 Aug 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2011 Jun 15;255(1):103–112. doi: 10.1016/j.taap.2011.06.005

Table 1.

Summary of nitrated proteins present in aniline-treated rats.

Spots no. ^*a	Protein Name	Accession no. ^*b	MW.(Da)	Identified Peptides no.	Identified Peptides Coverage %	MASCOT score ^*c	Theo. pI
1	Vinculin (Metavinculin)	gi\|157822133	123555.8	14	14	302	5.54
2	Vinculin, isoform CRA_b	gi\|149031250	116542.3	23	22	458	5.83
3	Eukaryotic translation elongation factor	gi\|8393296	95222.9	5	5	70	6.41
4	Eukaryotic translation elongation factor	gi\|8393296	95222.9	9	10	366	6.41
5	Eukaryotic translation elongation factor	gi\|8393296	95222.9	12	13	381	6.41
6	Ezrin	gi\|17902245	69362.6	6	9	152	5.83
7	Moesin, isoform CRA_a	gi\|149042266	67784.9	37	55	795	6.12
8	Moesin	gi\|149042266	67696.8	31	42	768	6.16
9	Transferrin	gi\|61556986	76314.5	8	12	386	6.94
10	Transferrin	gi\|61556986	76345.6	14	21	755	7.14
11	Transferrin	gi\|61556986	75809.4	13	21	542	7.57
12	Heat shock 90kDa protein 1, beta	gi\|40556608	83289.1	14	22	383	4.97
13	Heat shock 70kD protein 5	gi\|25742763	72302.4	33	54	1170	5.07
14	Lymphocyte cytosolic protein 1	gi\|58865656	70077.7	27	44	845	5.15
15	Heat shock protein 8	gi\|178847300	70827.2	28	53	960	5.37
16	Serum albumin precursor	gi\|124028612	68686.1	8	14	836	6.09
17	Serum albumin precursor	gi\|124028612	68686.1	18	27	987	6.09
18	Serum albumin precursor	gi\|124028612	68686.1	18	25	780	6.09
19	Serum albumin precursor	gi\|124028612	68686.1	21	29	866	6.09
20	Albumin	gi\|124028612	68686.1	21	27	872	6.09
21	Albumin	gi\|124028612	68686.1	21	27	931	6.09
22	Albumin	gi\|124028612	68686.1	18	32	812	6.09
23	Albumin	gi\|124028612	68686.1	13	31	101	6.09
24	5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase	gi\|2541906	64168.2	26	51	627	6.69
25	APEG-1 protein	gi\|1457952	12660.3	8	61	59	8.54
26	T-complex protein 1 subunit theta	gi\|34867525	59550.5	19	28	183	5.38
27	ATP synthase /F1-ATPase	gi\|149029718 /gi\|6729935	56309.5 51320.8	23 22	51	1080 1070	4.95 4.97
28	ARP3 (actin-related protein 3 homolog)	gi\|161728791	47327	15	34	465	5.61
29	Mitochondrial aldehyde dehydrogenase	gi\|25990263	53295.9	6	14	108	5.7
30	Mitochondrial aldehyde dehydrogenase	gi\|25990263	53295.9	15	30	583	5.7
31	Enolase 1, alpha	gi\|158186649	47086.2	17	50	903	6.16
32	Selenium binding protein	gi\|18266692	52498.4	15	35	235	6.1
33	Angiomotin	gi\|109510553	116787.9	21	63	55	7.06
39	Annexin A3 (Annexin-3)	gi\|122065130	36340.6	22	62	564	5.96
52	Lipocortin I (Annex A1)	gi\|235879	38794.9	11	34	401	6.97
53	Long-chain specific acyl-CoA dehydrogenase, mitochondrial precursor	gi\|6978431	47842.4	2	5	124	7.63
54	Gln synthetase	gi\|228136	40411.4	5	10	139	6.38
55	Isocitrate dehydrogenase 1	gi\|89573967	42628.3	7	19	133	5.98
56	Glutamate dehydrogenase 1, mitochondrial precursor	gi\|6980956	61377.3	4	7	169	8.05
57	Fibrinogen, B beta polypeptide	gi\|149048261	27423	2	7	71	8.71
58	Protein Vav 3 oncogene (predicted)	gi\|149025734	69319.3	10	16	57	8.98

^*a

Spot numbers are the same as shown in the 2D gel protein profiles of Fig. 2;

^*b

The accession numbers in NCBI database;

^*c

A MASCOT score >61 was considered significant (p<0.05) for proteins identified from data base.