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Accumulation of CBF1, CBF2, and CBF3 transcripts in different organs of Arabidopsis in response to low temperature. RNA-blot hybridizations were carried out with total RNA (15 μg per lane) isolated from leaves, stems, and roots of plants grown at 22°C (C) or exposed to 4°C for 1 h (4°). The specific probes for the CBF genes and the probe used for RBP4 are described in Methods.
