Elution pattern of NAS activity from the
gel-filtration column. The black circles indicate NAS activity. The
solid line indicates protein concentration monitored at 280 nm. The
active NAS fraction after hydroxyapatite chromatography was applied to
a Sephacryl S300HR column (1.5 × 71 cm, 125 mL). The column was
calibrated with standard proteins: thyroglobulin
(Mr 670,000), γ-globulin
(Mr 158,000), ovalbumin
(Mr 44,000), and myoglobin
(Mr 17,000). The linear flow was 10 cm
h−1.