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Purified wild-type MmeI in twofold serial dilutions from 16 to 1 µg on a 10–20% Tris–glycine gel (1 mm × 12 wells; Invitrogen, catalog No. EC61352) with an NEB 10–250 kDa protein ladder (NEB, catalog No. P7703S). The gel was run at 125 V constant voltage and stained using Coomassie Blue. The molecular weights of the two markers bracketing MmeI are 80 and 100 kDa.
