Table I.
Theoretical and measured Mrs of proteolytic fragments of carboxymethylated Fd I and Fd II
| Peak No.a | Fd I
|
Fd II
|
||||
|---|---|---|---|---|---|---|
| Peptideb | Observed value | Theoretical value | Peptideb | Observed value | Theoretical value | |
| 1 | Ala-1–Lys-6 | 694.3 | 694.8 | Ala-1–Lys-6 | 694.6 | 694.8 |
| 2 | Val-51–Ala-98 | 5224.3 | 5224.6 | Val-51–Leu-96 | 5041.6 | 5040.4 |
| 3 | Leu-7–Lys-50 | 4860.8 | 4861.1 | Leu-7–Lys-50 | 4879.0 | 4878.3 |
The observed Mrs of each of three polypeptides obtained by the digestion of Fd I and Fd II with lysylendopeptidase (shown in Fig. 2) were determined by LC/MS. The theoretical values of the polypeptides were calculated from the deduced amino acid sequences of pFD1 and pFD2.
a
Numbering of peaks is taken from Figure 2.
b
Numbering of amino acid residues is based on the sequence of the mature region of Fds shown in Figure 1C.