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. 2011 Dec 1;214(23):3977–3984. doi: 10.1242/jeb.061499

Fig. 2.

Fig. 2.

Semi-quantitative RT-PCR analysis. mRNA expression profile from two larvae (x and y) representative of knockdown and control groups collected at different time points (48, 72, 96 and 120 h) after first dsRNA feeding. (A) Verification of downregulation of IRS (GenBank accession no. XM_391985) expression in IRS– and IRS–/TOR– but not in TOR– larvae relative to control larvae. (B) TOR (XM_625127) expression was downregulated in TOR–, IRS– and IRS–/TOR– relative to control larvae. (C) ILP-1 (insulin-like peptide-1; GB_17332) expression was reduced in IRS–, TOR– and IRS–/TOR– larvae when compared with ILP-2. (D) ILP-2 (GB_10174) was expressed at higher levels in IRS– and TOR– larvae compared with ILP-1. (E) Actin (XM_623378) was used as a control gene. (F) PDK-1 (XM_396869) was up-regulated in TOR– larvae compared with the other groups. (G) PIP5K (XM_001120893) is up-regulated in IRS– and TOR– larvae when compared with controls. (H) PI3K (XM_394208) is initially down-regulated but later up-regulated after 96–120 h of dsRNA feeding in IRS– and TOR– larvae compared with controls. Increased transcription of PIP5K and PI3K may lead to greater production of diacylglycerol that promotes lipid storage (Zemel et al., 2000; Watt et al., 2003), a syndrome observed in IRS– larvae. General down-regulation of gene expression was observed in IRS–/TOR– larvae (B–F).