Human bronchial epithelial cells (Beas-2B) were treated with compound 2b (10 µM) in the presence of an antioxidant, N-acetyl cystiene (10 mM, NAC). Cells were harvested 24 h after the treatment, and the Nrf2-driven expression of NQO1, HO-1, and GCLM was quantified. β-actin was used for normalization. Data are representative of 3 independent experiments. Values shown are mean ± SD of triplicate wells (P ≤ 0.05).