Table 1. Data-collection statistics for Δ37N G6PDH.
Values in parentheses are for the highest resolution shell.
| Apoprotein | G6P–protein complex | |
|---|---|---|
| No. of crystals | 1 | 1 |
| Crystal dimensions (µm) | 50 × 50 × 200 | 50 × 50 × 100 |
| Beamline | ALS 5.0.2 | ALS 5.0.2 |
| Wavelength (Å) | 1.00 | 1.00 |
| Detector | ADSC Quantum 315 | ADSC Quantum 315 |
| Crystal-to-detector distance (mm) | 390 | 385 |
| Rotation range per image (°) | 0.5 | 0.5 |
| Total rotation range (°) | 170.5 | 167.5 |
| Exposure time per image (s) | 0.5 | 0.5 |
| Resolution range (Å) | 30.00–2.85 (3.00–2.85) | 67.36–3.35 (3.53–3.35) |
| Space group | P21 | P21 |
| Unit-cell parameters (Å, °) | a = 96.6, b = 133.0, c = 107.8, β = 100.1 | a = 96.8, b = 133.0, c = 107.7, β = 100.3 |
| Mosaicity (°) | 0.94 | 0.88 |
| Measured reflections | 214946 | 134881 |
| Unique reflections | 62028 | 38508 |
| Multiplicity | 3.5 (3.4) | 3.5 (3.6) |
| Mean I/σ(I) | 7.1 (2.3) | 5.5 (2.9) |
| Completeness (%) | 99.1 (99.2) | 99.5 (99.9) |
| Rmerge† (%) | 0.138 (0.501) | 0.209 (0.450) |
| Rmeas† (%) | 0.163 (0.594) | 0.246 (0.530) |
| Matthews coefficient‡ (Å3 Da−1) | 2.79 | 2.80 |
| Solvent content‡ (%) | 56.0 | 56.1 |
†
R
merge = 
and R
meas = 
, where 〈I(hkl)〉 is the average of symmetry- related (or Friedel-related) observations of a unique reflection.
‡
These figures correspond to the inclusion of four molecules in the asymmetric unit. In principle, a Matthews coefficient of 2.24 Å3 Da−1 (45% solvent) would be compatible with five protomers. Structure determination confirmed that a tetramer is the correct choice (see text).