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Purified trehalase from soybean nodules. One hundred nanograms of the the final elution (120 mm NaCl) from the Hi-Trap Q column step were separated on a 10% SDS-Laemmli gel and stained with silver. Sizes of the molecular mass markers are indicated in kD (lane M). The marker and the purified protein ran on the same gel, but an intervening lane was omitted.
