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. 2011 Nov 1;6(11):e26448. doi: 10.1371/journal.pone.0026448

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A The expression level of ten selected miRNAs was in 3 developmental stages of T. spiralis measured by qRT-PCR. GAPDH was chosen as an endogenous reference. B Identification of the miRNAs by northern-blot. Lanes from left to right: new born larvae (NBL), muscle larvae (ML) and adult (Ad). The 5S rRNA bands were visualized by ethidium bromide staining of polyacrylamide gels and served as loading controls (bottom).