Table 1. Conserved (common) miRNAs identified in different developmental stages.
| Name | Mature Arm | miR*a | Most abundant sequence | Length | Expression (TPMb) | ||
| Ad | NBL | ML | |||||
| tsp-miR-228 | 5′ | Y | AAUGGCACUGGAUGAAUUCACGG | 23 | 23725 | 19859 | 60017 |
| tsp-miR-100 | 5′ | Y | AACCCGUAGAUCCGAACUUGUGU | 23 | 3028 | 35995 | 541 |
| tsp-let-7 | 3′ | N | UGAGGUAGUAGGUUGUAUAGUU | 22 | 25573 | 1067 | 5554 |
| tsp-miR-1 | 3′ | Y | UGGAAUGUAAAGAAGUAUGUAG | 22 | 1515 | 9759 | 3351 |
| tsp-miR-31 | 5′ | N | AGGCAAGAUGUUGGCAUAGCUGA | 23 | 1004 | 7728 | 2025 |
| tsp-miR-125 | 5′ | Y | UCCCUGAGACCCAAACUUGUGA | 22 | 745 | 10 | 414 |
| tsp-miR-252 | 5′ | Y | CUAAGUAGUAGUGCCGCAGGUC | 22 | 193 | 279 | 290 |
| tsp-miR-9-1 | 5′ | Y | UCUUUGGUUAUCUAGCUGUAUGA | 23 | 239 | 203 | 179 |
| tsp-miR-87 | 3′ | N | GUGAGCAAAGUUUCAGGUGUGU | 22 | 78 | 160 | 120 |
| tsp-miR-9-2 | 3′ | Y | AUAAGCUAGUUGACCAAAGA | 20 | 47 | 25 | 97 |
| tsp-miR-29 | 3′ | Y | UAGCACCAUUUGAAUUCAGUG | 21 | 21 | 8 | 24 |
| tsp-miR-9-3 | 3′ | Y | UAAAGCUGGAUGACCAAAGU | 20 | 18 | 8 | 26 |
| tsp-miR-993 | 3′ | Y | GAAGCUCGUUUCUACAGG | 18 | 5 | 5 | 16 |
| tsp-miR-133 | 5′ | Y | ACUGGUUGAGGACGUACCAAAUUG | 24 | 1 | 3 | 1 |
| tsp-miR-34 | 5′ | Y | UGGCAGUGUAAUUAGCUGGUUGU | 23 | 1 | 1 | 2 |
a
Y indicates that sequences from both arms of a pre-miRNA species were found, while N means that only a sequence from one arm was identified.
b
The abundance value of each miRNA was normalized to “transcripts per million (TPM)”. If the value after normalization was less than 1, the normalized value was set as 1.
doi:10.1371/journal.pone.0026448.t001