TABLE 1.
Yields of biomass and metabolites in the stages of lysine production by Corynebacterium glutamicum ATCC 21526 from glucose or fructosea
| Yield | Lysine production on:
|
|
|---|---|---|
| Glucose | Fructose | |
| Biomass | 54.1 ± 0.8 | 28.5 ± 0.0 |
| Lysine | 281.0 ± 2.0 | 244.4 ± 23.3 |
| Valine | 0.1 ± 0.0 | 0.0 ± 0.0 |
| Alanine | 0.1 ± 0.0 | 0.4 ± 0.1 |
| Glycine | 6.6 ± 0.0 | 7.1 ± 0.4 |
| Dihydroxyacetone | 26.3 ± 15.3 | 156.6 ± 25.8 |
| Glycerol | 3.8 ± 2.4 | 38.4 ± 3.9 |
| Trehalose | 3.3 ± 0.5 | 0.9 ± 0.1 |
| α-Ketoglutarate | 1.6 ± 0.4 | 6.5 ± 0.3 |
| Acetate | 45.1 ± 0.3 | 36.2 ± 5.7 |
| Pyruvate | 1.2 ± 0.4 | 2.1 ± 0.5 |
| Lactate | 7.1 ± 1.7 | 38.3 ± 3.5 |
a
The yields are the mean values of two parallel incubations on (i) 40 mM 1-13C-labeled substrate and (ii) 20 mM 13C6-labeled substrate plus 20 mM naturally labeled substrate with the corresponding deviations between the two incubations. All yields are given in mmol of product mol−1 except the yield for biomass, which is given in mg of dry biomass mmol−1.