Figure 2. Lack of TLR2/4 does not impair pulmonary recruitment of CD4⁺ and CD8⁺ T-cells upon infection with C. pneumonia.

Wild type (n = 3/time point) and TLR2/4 double-deficient mice (n = 3/time point) were infected with C. pneumoniae. At the time points indicated in the graph mice were sacrificed, lungs removed, single cell suspensions were prepared and the number of cells determined. Cells were stained with mAbs specific for CD4, CD8 and CD3 as described in Materials and Methods, analyzed by flow cytometry and the number of each subpopulation was calculated. CD4⁺CD3⁺ T-cells are depicted in (A), CD8⁺CD3⁺ T-cells in (B). Error bars represent SD of three individual mice.