Figure 7. The roles of epidermal growth factor-like motifs of versican G3 domain in modulating breast cancer cell apoptosis induced by chemotherapeutic drugs.

a)1×10⁴ G3-, G3ΔEGF- and vector-transfected 4T07 were inoculated and cultured in 10% FBS/DMEM medium in 96 well culture dishes for 12 hours. After cell attachment, cells were treated with 40 µM C2-ceramide, 2 µM Docetaxel, 8 µM Doxorubicin, or 10 µM Epirubicin for 24 hours. Cell viability was tested by WST-1 assays. b) After treated with 2 µM Docetaxel or 8 µM Doxorubicin for 1 hour, samples were subjected to Annexin V assays. c) Treated with 2 µM Docetaxel or 8 µM Doxorubicin all cells were processed to lysates and subjected to immunoblotting with antibodies to pSAPK/JNK, SAPK/JNK, ERK2, pERK, GSK-3β (S9P), and β-actin.