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. 2004 Jan;70(1):96–103. doi: 10.1128/AEM.70.1.96-103.2004

FIG. 4.

FIG. 4.

Overexpression of the Mur-LH endolysin and demonstration of its lytic activity in a zymogram. SDS-PAGE was performed on E. coli BL21 harboring nonrecombinant plasmid pET (lane 2) and on E. coli BL21 harboring recombinant plasmid pET-(lys) expressing the endolysin of bacteriophage φ-0303 (lane 3). Lanes 4 and 5 contain the same samples in lanes 2 and 3, respectively, loaded on a zymogram with autoclaved cells of L. helveticus CNRZ 892 as a substrate. Lane 1 contains molecular mass markers. The positions of the molecular mass markers (in kilodaltons) are indicated to the left of the gel.