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. 2011 Nov 9;6(11):e26682. doi: 10.1371/journal.pone.0026682

Figure 5. Both maternal environment and strain influenced mRNA expression associated with fetal and perinatal lung maturation.

Figure 5

Quantitative RT-PCR for selected genes was performed to determine lung mRNA levels at E17.5 (A) and PN1 (B) from AJ-OT mice compared to mice from normal A/J dams. At E17.5, Napsa, Eln, Fabp5, Scnn1g, Sftpc, and Lpcat1 mRNA levels were significantly induced in AJ-OT lungs. A subset of mRNAs, including Abca3, Eln, Flt4, Lyz2, Sftpc, Slc34a2, and Tek, was expressed at higher levels in the AJ-OT mice on PN1 compared to normal A/J mice. Results were normalized to 18S rRNA and expressed as the means ± SEM of 12 animals per group; *p< 0.05, **p< 0.01, ***p< 0.001 for AJ-OT higher than A/J and ♦p< 0.05, ♦♦p< 0.01, ♦♦♦p< 0.001 for AJ-OT lower than A/J.