Figure 6. XRFC regulates neural crest gene expression through the one-carbon metabolism pathway.

Co-injection of 5-MTHF (20 ng/embryo, E–H), SAM (10 ng/embryo, I–L) or Vitamin B12 (50 ng/embryo, M–P) with XRFC-MO rescued the expression of the neural crest maker genes (compare with A–D). (Q) Quantification of the rescue of the XRFC-MO effect by 5-MTHF, SAM, and Vitamin B12.