Figure 4. IgE ELISA inhibition of kidney bean extract using self protein as inhibitor.

Kidney bean positive patients' pooled sera (1∶10 v/v) was preincubated with 5, 10, 50, 100, 1000, 10000 ng of three batches of kidney bean extract as inhibitors (Kb1, Kb2, Kb3), bromelain and CL (Curvularia lunata) were also used as inhibitor for IgE inhibition to kidney bean. ELISA was carried out on solid phase coated kidney bean (2 µg/100 µl/well) and preincubated sera.