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. 2011 Nov 9;6(11):e27193. doi: 10.1371/journal.pone.0027193

Table 5. Peptide mass fingerprint database search of selected spots from 1-DE of kidney bean proteins.

Spot No. Accession no. Identification Matches (Ion score >50)a Sequence Coverageb (%) MASCOT scorec Tht mass/pI (kDa) Obs mass (kDa) Proposed function
1 CAH60259 Alpha-amylase inhibitor precursor [Phaseolus coccineus] 17(9) 22 256 27.0/5.17 18 defensive protein
2 P05088 Full = Erythroagglutinating phytohemagglutinin; AltName: Full = PHA-E 39(21) 32 593 29.7/5.15 34 defensive protein
3 CAD29133 lectin [Phaseolus vulgaris] 19(7) 21 278 29.6/4.83 37 defensive protein
4 CAD29133 Lectin [Phaseolus vulgaris]. 54(32) 26 494 29.6/4.83 40 defensive protein
5 AAA99534 Phaseolin [Phaseolus vulgaris] 32(19) 32 931 47.5/5.42 42 storage protein
6 P07219 Full = Phaseolin, alpha-type 34(17) 26 766 49.2/5.25 45 storage protein
7 ABA26579 Group 3 late embryogenesis abundant protein [Phaseolus vulgaris] 7(1) 16 328 50.7/5.80 50 Stress tolerant protein
8 ABA26579 Group 3 late embryogenesis abundant protein [Phaseolus vulgaris] 4(2) 6 130 50.7/5.80 58 Stress tolerant protein

Footnotes: Abbreviations; Tht  =  Theoretical, Obs  =  Observed. aMatches a  =  Number of peptides matched with protein in MS/MS query. bSequence coverage b  =  Total percentage of proteins amino acid sequence covered by peptides in MS/MS analysis. cMASCOT score c =  >40 indicate identification or extensive homology (p<0.05).