Table 5. Peptide mass fingerprint database search of selected spots from 1-DE of kidney bean proteins.
Spot No. | Accession no. | Identification | Matches (Ion score >50)a | Sequence Coverageb (%) | MASCOT scorec | Tht mass/pI (kDa) | Obs mass (kDa) | Proposed function |
1 | CAH60259 | Alpha-amylase inhibitor precursor [Phaseolus coccineus] | 17(9) | 22 | 256 | 27.0/5.17 | 18 | defensive protein |
2 | P05088 | Full = Erythroagglutinating phytohemagglutinin; AltName: Full = PHA-E | 39(21) | 32 | 593 | 29.7/5.15 | 34 | defensive protein |
3 | CAD29133 | lectin [Phaseolus vulgaris] | 19(7) | 21 | 278 | 29.6/4.83 | 37 | defensive protein |
4 | CAD29133 | Lectin [Phaseolus vulgaris]. | 54(32) | 26 | 494 | 29.6/4.83 | 40 | defensive protein |
5 | AAA99534 | Phaseolin [Phaseolus vulgaris] | 32(19) | 32 | 931 | 47.5/5.42 | 42 | storage protein |
6 | P07219 | Full = Phaseolin, alpha-type | 34(17) | 26 | 766 | 49.2/5.25 | 45 | storage protein |
7 | ABA26579 | Group 3 late embryogenesis abundant protein [Phaseolus vulgaris] | 7(1) | 16 | 328 | 50.7/5.80 | 50 | Stress tolerant protein |
8 | ABA26579 | Group 3 late embryogenesis abundant protein [Phaseolus vulgaris] | 4(2) | 6 | 130 | 50.7/5.80 | 58 | Stress tolerant protein |
Footnotes: Abbreviations; Tht = Theoretical, Obs = Observed. aMatches a = Number of peptides matched with protein in MS/MS query. bSequence coverage b = Total percentage of proteins amino acid sequence covered by peptides in MS/MS analysis. cMASCOT score c = >40 indicate identification or extensive homology (p<0.05).