TABLE 3.
Identified fatty acid | Mean ± SEM % (wt/wt) fatty acid in L. bulgaricus grown in MRS broth supplemented with:
|
|||||
---|---|---|---|---|---|---|
Nothing (control) | 18:2 n-6 | 18:3 n-6 | 20:4 n-6 | 18:3 n-3 | 22:6 n-3 | |
SAFA | 13.8 ± 1.4 | 13.3 ± 0.6 | 14.2 ± 1.2 | 13.6 ± 0.7 | 13.9 ± 0.2 | 17.0 ± 0.2* |
18:1 n-9 | 29.0 ± 3.9 | 34.3 ± 1.7 | 39.2 ± 2.4* | 37.0 ± 1.9 | 27.5 ± 0.5 | 29.7 ± 0.5 |
18:1 n-7 | 3.5 ± 0.1 | 3.3 ± 0.1* | 3.1 ± 0.2 | 3.4 ± 0.0 | 2.8 ± 0.0* | 3.4 ± 0.0 |
18:2 n-6 | ND | 3.5 ± 0.8* | ND | ND | ND | ND |
18:3 n-6 | 0.5 ± 0.2 | 0.8 ± 0.4 | 2.1 ± 0.6* | 0.4 ± 0.1 | 0.6 ± 0.2 | 0.5 ± 0.1 |
20:4 n-6 | ND | ND | ND | 0.4 ± 0.0* | ND | ND |
cis-9,10Mt 18:0 and 18:3 n-3b | 30.3 ± 0.2 | 27.7 ± 0.3 | 21.6 ± 3.5 | 26.6 ± 1.5 | 35.6 ± 0.7* | 29.2 ± 0.7 |
cis-11,12Mt 18:0 | ND | ND | ND | ND | ND | ND |
20:5 n-3 | 1.4 ± 0.6 | 1.0 ± 0.1 | 1.1 ± 0.3 | 1.0 ± 0.1 | 0.7 ± 0.1 | 0.7 ± 0.1 |
22:6 n-3 + 24:1b | 1.7 ± 0.3 | 1.3 ± 0.4 | 1.1 ± 0.3 | 1.2 ± 0.4 | 1.9 ± 0.3 | 1.7 ± 0.3 |
18:2 c-9,t-11 and t-8, c-10b | 1.4 ± 0.2 | 1.1 ± 0.0 | 0.9 ± 0.2 | 1.1 ± 0.0 | 2.0 ± 0.0* | 1.9 ± 0.0* |
18:2 t-10,c-12 and c-11,t-13b | 1.3 ± 0.3 | 1.1 ± 0.0 | 0.8 ± 0.2 | 1.1 ± 0.1 | 1.9 ± 0.0** | 1.9 ± 0.0* |
The PUFA concentration was 5 μg/ml. Six independently prepared fatty acid methyl esters were analyzed in duplicate. *, P < 0.05; **, P < 0.07. SAFA, 14:0 + 16:0 + 18:0 + 20:0 + 22:0; ND, not detected.
Overlapping peaks in the chromatograms.