TABLE 3.

Effect of various free PUFA in growth medium on fatty acid compositions of total-lipids of L. delbrueckii subsp. bulgaricus^a

Identified fatty acid	Mean ± SEM % (wt/wt) fatty acid in L. bulgaricus grown in MRS broth supplemented with:
	Nothing (control)	18:2 n-6	18:3 n-6	20:4 n-6	18:3 n-3	22:6 n-3
SAFA	13.8 ± 1.4	13.3 ± 0.6	14.2 ± 1.2	13.6 ± 0.7	13.9 ± 0.2	17.0 ± 0.2*
18:1 n-9	29.0 ± 3.9	34.3 ± 1.7	39.2 ± 2.4*	37.0 ± 1.9	27.5 ± 0.5	29.7 ± 0.5
18:1 n-7	3.5 ± 0.1	3.3 ± 0.1*	3.1 ± 0.2	3.4 ± 0.0	2.8 ± 0.0*	3.4 ± 0.0
18:2 n-6	ND	3.5 ± 0.8*	ND	ND	ND	ND
18:3 n-6	0.5 ± 0.2	0.8 ± 0.4	2.1 ± 0.6*	0.4 ± 0.1	0.6 ± 0.2	0.5 ± 0.1
20:4 n-6	ND	ND	ND	0.4 ± 0.0*	ND	ND
cis-9,10Mt 18:0 and 18:3 n-3b	30.3 ± 0.2	27.7 ± 0.3	21.6 ± 3.5	26.6 ± 1.5	35.6 ± 0.7*	29.2 ± 0.7
cis-11,12Mt 18:0	ND	ND	ND	ND	ND	ND
20:5 n-3	1.4 ± 0.6	1.0 ± 0.1	1.1 ± 0.3	1.0 ± 0.1	0.7 ± 0.1	0.7 ± 0.1
22:6 n-3 + 24:1b	1.7 ± 0.3	1.3 ± 0.4	1.1 ± 0.3	1.2 ± 0.4	1.9 ± 0.3	1.7 ± 0.3
18:2 c-9,t-11 and t-8, c-10b	1.4 ± 0.2	1.1 ± 0.0	0.9 ± 0.2	1.1 ± 0.0	2.0 ± 0.0*	1.9 ± 0.0*
18:2 t-10,c-12 and c-11,t-13b	1.3 ± 0.3	1.1 ± 0.0	0.8 ± 0.2	1.1 ± 0.1	1.9 ± 0.0**	1.9 ± 0.0*

^aThe PUFA concentration was 5 μg/ml. Six independently prepared fatty acid methyl esters were analyzed in duplicate. *, P < 0.05; **, P < 0.07. SAFA, 14:0 + 16:0 + 18:0 + 20:0 + 22:0; ND, not detected.

^bOverlapping peaks in the chromatograms.