TABLE 4.

Effect of various free PUFA in growth medium on fatty acid compositions of total-lipids of L. casei Shirota^a

Identified fatty acid	Mean ± SEM % (wt/wt) fatty acid in L. casei Shirota grown in MRS supplemented with:
	Nothing (control)	18:2 n-6	18:3 n-6	20:4 n-6	18:3 n-3	22:6 n-3
SAFA	13.5 ± 1.3	13.4 ± 0.4	13.9 ± 0.6	11.9 ± 1.0	11.0 ± 0.6	12.1 ± 1.0
18:1 n-9	32.4 ± 1.1	25.1 ± 0.9*	28.6 ± 0.2*	31.7 ± 1.3	30.5 ± 0.8	33.3 ± 0.8
18:1 n-7	3.1 ± 0.0	2.8 ± 0.0*	2.8 ± 0.0*	3.1 ± 0.0	2.9 ± 0.0*	3.1 ± 0.0
18:2 n-6	ND	4.6 ± 0.0*	ND	ND	ND	ND
18:3 n-6	0.4 ± 0.1	0.5 ± 0.0	3.1 ± 0.5*	0.4 ± 0.1	0.6 ± 0.3	0.4 ± 0.1
20:4 n-6	ND	ND	ND	0.5 ± 0.0*	ND	ND
cis-9, 10Mt 18:0 and 18:3 n-3b	35.3 ± 1.0	31.6 ± 1.8	31.1 ± 1.1*	37.5 ± 0.2	40.3 ± 0.4*	36.2 ± 0.4
cis-11,12Mt 18:0	ND	ND	ND	ND	ND	ND
20:5 n-3	0.8 ± 0.0	0.9 ± 0.0*	0.9 ± 0.0*	0.8 ± 0.0	0.8 ± 0.0	0.8 ± 0.0
22:6 n-3 + 24:1b	1.4 ± 0.1	1.9 ± 0.4	1.8 ± 0.2	1.1 ± 0.1	1.2 ± 0.1	1.0 ± 0.2
18:2 c-9,t-11 and t-8,c-10b	1.7 ± 0.1	1.9 ± 0.1	1.9 ± 0.1	1.6 ± 0.0	1.5 ± 0.0*	1.6 ± 0.0
18:2 t-10,c-12 and c-11,t-13b	1.7 ± 0.1	1.9 ± 0.1	2.0 ± 0.1**	1.5 ± 0.0	1.4 ± 0.0*	1.5 ± 0.0

^aThe PUFA concentration was 5 μg/ml. Six independently prepared fatty acid methyl esters were analyzed in duplicate. *, P < 0.05; **, P < 0.07. SAFA, 14:0 + 16:0 + 18:0 + 20:0 + 22:0; ND, not detected.

^bOverlapping peaks in the chromatograms.