TABLE 5.

Effect of various free PUFA in growth medium on bacterial cell surface properties, measured by using the MATS method (1)^a

Bacterium	PUFA supplementation	PUFA concn (μg/ml)	Mean ± SEM % affinity for:
			Chloroform	Tetradecane	Ethyl acetate	Octane
Lactobacillus GG	None (control)	0	15 ± 1	8 ± 0	3 ± 1	17 ± 3
	18:2 n-6	5	10 ± 4	13 ± 3	2 ± 3	12 ± 2
		20	12 ± 1	13 ± 4	0 ± 1	11 ± 3
	18:3 n-6	5	10 ± 2	10 ± 2	1 ± 1	10 ± 0
		20	9 ± 6	7 ± 1	1 ± 2	8 ± 2b
	20:4 n-6	5	4 ± 1	8 ± 2	0 ± 1	11 ± 2
		20	8 ± 4	5 ± 1	1 ± 2	12 ± 2
	18:3 n-3	5	9 ± 2	10 ± 4	2 ± 1	10 ± 3
		20	5 ± 2	15 ± 2	4 ± 2	14 ± 1
	22:6 n-6	5	6 ± 1b	7 ± 1	1 ± 3	13 ± 3
		20	7 ± 8	16 ± 6	0 ± 3	12 ± 4
L. delbrueckii subsp. bulgaricus	None (control)	0	13 ± 3	14 ± 2	3 ± 2	15 ± 2
	18:2 n-6	5	9 ± 3	20 ± 6	1 ± 1	15 ± 4
		20	15 ± 5	17 ± 1	2 ± 1	11 ± 2
	18:3 n-6	5	9 ± 2	22 ± 12	2 ± 1	12 ± 3
		20	10 ± 2	11 ± 2	1 ± 1	12 ± 4
	20:4 n-6	5	8 ± 3	19 ± 10	0 ± 0	15 ± 1
		20	7 ± 0	10 ± 0	0 ± 0	8 ± 2
	18:3 n-3	5	11 ± 3	25 ± 14	2 ± 2	14 ± 1
		20	13 ± 4	10 ± 2	2 ± 2	22 ± 4
	22:6 n-6	5	8 ± 2	9 ± 3	1 ± 1	15 ± 2
		20	9 ± 2	10 ± 3	1 ± 1	24 ± 8
L. casei Shirota	None (control)	0	11 ± 1	12 ± 0	2 ± 2	15 ± 1
	18:2 n-6	5	7 ± 0b	14 ± 2	1 ± 1	11 ± 0b
		20	6 ± 2	16 ± 2	2 ± 0	14 ± 3
	18:3 n-6	5	5 ± 0b	9 ± 2	0 ± 1	10 ± 1b
		20	11 ± 1	12 ± 1	2 ± 2	13 ± 2
	20:4 n-6	5	6 ± 2	10 ± 4	2 ± 2	8 ± 1b
		20	10 ± 3	10 ± 1	2 ± 2	14 ± 3
	18:3 n-3	5	5 ± 1	13 ± 1	0 ± 1	10 ± 2
		20	10 ± 2	15 ± 1	3 ± 1	12 ± 2
	22:6 n-6	5	8 ± 2	13 ± 1	1 ± 2	10 ± 1b
		20	12 ± 3	9 ± 1	0 ± 3	13 ± 2

^aThe two nonpolar solvents (tetradecane and octane) were used to estimate the hydrophobicity properties of the lactobacilli, whereas the two polar solvents (chloroform and ethyl acetate) were selected for the estimation of the Lewis acid/base (i.e., electron donor/electron acceptor) character.

^bP < 0.05, as determined by paired t test.