TABLE 1.

16S rRNA-targeted oligonucleotide probes used in this study^a

Probe name	Sequencec	Target organisms	Target site (E. coli positions)	% FA	NaCl concn (mM)	Reference(s)
UNI	5′-GWA TTA CCG CGG CKG CTG-3′	Universal	519-536	20	70	20
EUB338	5′-GCT GCC TCC CGT AGG AGT-3′	Domain bacteria	338-355	20	225	3, 42
NON338	5′-ACT CCT ACG GGA GGC AGC-3′	Negative control	NAb	20	225	46
NSO190	5′-CGA TCC CCT GCT TTT CTC C-3′	β-AOB	190-208	55	20	28, 42
NSO1225	5′-CGC GAT TGT ATT ACG TGT GA-3′	β-AOB	1225-1244	35	191	28, 42

^aProbes are shown together with the target organisms, target sites on the 16S rRNA gene with respect to Escherichia coli positions, formamide concentrations (% FA) in hybridization solutions, and NaCl concentrations in the stringent washing solutions.

^bNA, not applicable.

^cW = A or T; K = G or T.