TABLE 1.
Cofactor requirements for NAD+ reduction in cell extract of cyclopropanecarboxylate-grown R. rhodochrous cells
| Missing componenta | Amt of NADH formed (nmol/min/mg of protein) |
|---|---|
| None | 3.0 |
| Cyclopropanecarboxylate | 0.0 |
| CoA | 0.1 |
| ATP | 0.1 |
| NAD+ | 0.0 |
| Extract | 0.0 |
a
The complete system contained 10 mM cyclopropanecarboxylate, 1 mM CoA, 1 mM ATP, 1 mM MgCl2, 1 mM NAD, 25 mM potassium phosphate buffer (pH 7.2), and 12 mg of protein in the extract in a total volume of 1.0 ml. The rate of NADH formation was determined as described in the text.