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. 2004 Jan;70(1):224–228. doi: 10.1128/AEM.70.1.224-228.2004

TABLE 2.

TLC analyses of the metabolic intermediates trapped as hydroxamic acid derivativea

Hydroxamic acid Rf value in solvent:
A B C
Hydroxamic acid I 0.75 0.59 0.69
Hydroxamic acid II 0.59 0.31 0.42
Cyclopropanecarboxohydroxamic acid 0.76 0.59 0.68
3-Hydroxybutyrohydroxamic acid 0.60 0.33 0.43
Methacrylohydroxamic acid 0.79
dl-3-Hydroxyisobutyrohydroxamic acid 0.64 0.42 0.49
a

Experimental details are described in the text. Solvent systems used for silica gel TLC were as follows: A, water-saturated 2-butanol; B, 2-butanol containing 1% CH3COOH; and C, 2-butanol containing 1% NH4OH.