TABLE 3.
Reduction of NAD+ with CoA thioesters and corresponding carboxylic acids in cell extract of cyclopropanecarboxylate-grown cellsa
| Substrate | Amt of NADH formed (nmol/min/mg of protein)
|
|
|---|---|---|
| Without CoA and ATP | With CoA and ATP | |
| None | 0 | 0 |
| Cyclopropanecarboxyl-CoA | 3.3 | |
| 3-Hydroxybutyryl-CoA | 26.6 | |
| Crotonyl-CoA | 8.1 | |
| Methacrylyl-CoA | 0.0 | |
| 3-Hydroxyisobutyryl-CoA | 0.1 | |
| Cyclopropanecarboxylate | 2.6 | |
| 3-Hydroxybutyrate | 8.8 | |
| Crotonate | 27.3 | |
| Methacrylate | 0.1 | |
| 3-Hydroxyisobutyrate | 0.1 | |
| 2-Hydroxybutyrate | 0.0 | |
a
Specificities for organic acid substrates and CoA thioester substrates were determined in the presence and absence of 1 mM CoA plus 1 mM ATP, respectively.