TABLE 4.

Reduction of NAD⁺ by CoA thioesters and corresponding carboxylic acids in cell extract of citrate-grown cells^a

Substrate	Amt of NADH formed (nmol/min/mg of protein)
	Without CoA and ATP	With CoA and ATP
None	0	0
Cyclopropanecarboxyl-CoA	0.0
3-Hydroxybutyryl-CoA	4.8
Crotonyl-CoA	4.7
Cyclopropanecarboxylate		0.0
3-Hydroxybutyrate		0.3
Crotonate		1.0

^aAssay conditions were identical to those described in Table 3, footnote a.