TABLE 2.
Sequences of oligonucleotide primers used in PCRs and expected product sizes
| Primera | Sequence | Size (bp) of amplicon | Source or reference |
|---|---|---|---|
| prtP (F) | 5′-CGGTTATTGACAAGTGGC-3′ | 1,200 | 17 |
| prtP (R) | 5′-CGAGGCCATTGACCGTACC-3′ | 41 | |
| PLL+1 (F) | 5′-ATGGCATGCAAACATCTTACCATAGACGAA-3′ | 22,910 | 7 |
| PLL-23 (R) | 5′-TTCAGGATCCACGCAAAGACTAACCAAATA-3′ | ||
| PLL+40 (F) | 5′-ATCGTGTCGACTGGAGTGAATTTATTGTTA-3′ | 23,108 | 7 |
| PLL-4 (R) | 5′-CATTATCAGCTGAAAATTCTTGTCGACTGA-3′ | ||
| p24850 (F) | 5′-AAGGGAATTGAAGGGGTTCTGTAC-3′ | 12,666 | 7 |
| p37500 (R) | 5′-TCTTAAGTATAGGGCAA-3′ | ||
| p28000 (F) | 5′-ACAATTGGGAAAATACCTTGAAGAT-3′ | 3,281 | 7 |
| p31261 (R) | 5′-CTTGACCCATCTCTACCTGGTTCCT-3′ | ||
| PLL+40 (F) | 5′-ATCGTGTCGACTGGAGTGAATTTATTGTTA-3′ | 5,444 | 7 |
| p45500 (R) | 5′-TCCGACTCCACATACAAT-3′ | ||
| p46000 (F) | 5′-ACTTTGCCTGCAGTCTGAGCG-3′ | 5,489 | 7 |
| p51500 (R) | 5′-GATAAATTGGGATATTTAAGG-3′ | ||
| p51400 (F) | 5′-TCTGATAGGATCGCCTTAAG-3′ | 3,146 | 7 |
| p54500 (R) | 5′-TGTTGGTTGAAAAATTGTTG-3′ | ||
| p54400 (F) | 5′-TAAATTATAGCGATTGGTTG-3′ | 3,054 | 7 |
| p57500 (R) | 5′-TCTATCAGTGGTTAGCAAAG-3′ | ||
| p51400 (F) | 5′-TCTGATAGGATCGCCTTAAG-3′ | 6,106 | 7 |
| p57500 (R) | 5′-TCTATCAGTGGTTAGCAAAG-3′ | ||
| p54400 (F) | 5′-TAAATTATAGCGATTGGTTG-3′ | 8,912 | 7 |
| PLL-4 (R) | 5′-CATTATCAGCTGAAAATTCTTGTCGACTGA-3′ | ||
| PLL+1 (F) | 5′-ATGGCATGCAAACATCTTACCATAGACGAA-3′ | 2,853 | 7 |
| PLL-4 (R) | 5′-CATTATCAGCTGAAAATTCTTGTCGACTGA-3′ | ||
| p18150 (F) | 5′-AAGTGAGTGAAGAAGTCC-3′ | 4,850 | 7 |
| PLL-23 (R) | 5′-TTCAGGATCCACGCAAAGACTAACCAAATA-3′ | ||
| p21590 (F) | 5′-CAGTTGATAGTTATTTAG-3′ | 1,420 | 7 |
| PLL-23 (R) | 5′-TTCAGGATCCACGCAAAGACTAACCAAATA-3′ | ||
| p23800 (F) | 5′-GTTGTGAGCTGGTTTTTC-3′ | 7,450 | 7 |
| p31261 (R) | 5′-CTTGACCCATCTCTACCTGGTTCCT-3′ | ||
| p24270 (F) | 5′-CGGTTCAATGCTCAGTTT-3′ | 7,011 | 7 |
| p31261 (R) | 5′-CTTGACCCATCTCTACCTGGTTCCT-3′ | ||
| p37510 (F) | 5′-TTGCCCTATACTTAAGA-3′ | 4,782 | 7 |
| p42270 (R) | 5′-CCATGAGACCCTCTTACG-3′ | ||
| p37510 (F) | 5′-TTGCCCTATACTTAAGA-3′ | 2,598 | 7 |
| p40000 (R) | 5′-TAACAATAAATTCACTCC-3′ | ||
| p37510 (F) | 5′-TTGCCCTATACTTAAGA-3′ | 3,679 | 7 |
| p41170 (R) | 5′-CTGCCCTCGGAGTTCTAA-3′ | ||
| MT60A J1 (F) | 5′-TTTCACATTCCTCATTATAGAGTT-3′ | 1,673 | This study |
| p25021 J1 (R) | 5′-ATTGGCATGTTGACATTT-3′ | This study | |
| J2 280 J2 (F) | 5′-AGGACATACATATAGACA-3′ | 855 | This study |
| J2 1120 J2 (R) | 5′-AAGTCCTTTTTACTTTAT-3′ | This study | |
| J2 280 (F) | 5′-AGGACATACATATAGACA-3′ | 2,021 | 7 |
| p42270 (R) | 5′-CCATGAGACCCTCTTACG-3′ | ||
| p40081 | 5′-ATCGTTCCGACTGGAGTGAATTTATT-3′ | Inverse PCR | 7 |
| p38890 | 5′-TGTAGATGAGATGGCAACAATAACTG-3′ | ||
| p24270 | 5′-ACATTAAAACTGAGCATTGAACCG-3′ | Inverse PCR | 7 |
| p24850 | 5′-AAGGGAATTGAAGGGGTTCTGTAC-3′ |
a
F, forward; R, reverse.