Fig. 1.

Deletion of dns and xds results in an increase of biofilm formation. Biofilms of the wild type (WT), deletion mutants, mutants and WT with empty pMMB plasmid or complemented mutants, as indicated, were quantified after 12 h (A), 24 h (B), 40 h (C), 48 h (D) and 72 h (E). The time points are indicated on top of each panel. The biofilm formation capacity was assayed under static conditions by crystal violet staining and subsequent determination of the OD₅₉₅. Shown are the medians from at least eight independent measurements. The error bars indicate the interquartile range. Significant differences (*P < 0.05) are indicated for the following comparisons: deletion mutants with wild type; ΔdnsΔxds (pdns), ΔdnsΔxds (pxds) and ΔdnsΔxdsΔampD (pMMB) with ΔdnsΔxds (pMMB); ΔdnsΔxdsΔampD (pMMB) with ΔdnsΔxdsΔampD (pampD); Δdns (pdns) with Δdns (pMMB); and Δxds (pxds) with Δxds (pMMB).