Table 1.
Strains used in this study
| Strain/plasmid | Description | Reference |
|---|---|---|
| E. coli | ||
| DH5αλpir | F-Φ80ΔlacZΔM15Δ(argF lac)U169 deoR recA1 endA1 hsdR17 (rK-mK+) supE44 thi-1 gyrA69 relA1, λpirR6K, Apr | Hanahan (1983) |
| SM10λpir | thi thr leu tonA lacY supE recA::RPA-2-Te::Mu λpirR6K, Kmr | Miller and Mekalanos (1988) |
| V. cholerae | ||
| C6709 | WT, O1 El Tor Inaba, clinical isolate, 1991 Peru, tcpA+ctx+hapR+, spontaneous Smr | Roberts et al. (1992) |
| C6709lacZ | C6709, lacZ::res-neo-sacB-res | Tamayo et al. (2008) |
| Δdns | Deletion of dns in C6709, Smr | This study |
| ΔhapR | Deletion of hapR in C6709, Smr | This study |
| ΔvpsA | Deletion of vpsA in C6709, Smr | This study |
| Δxds | Deletion of xds in C6709, Smr | This study |
| ΔdnsΔxds | Deletion of xds in Δdns, Smr | This study |
| ΔdnsΔxdsΔampD | Deletion of in ampD in ΔdnsΔxds, Smr | This study |
| ΔdnsΔxds lacZ | ΔdnsΔxds, lacZ::res-neo-sacB-res | This study |
| C6709dns::pGPphoA | Insertion of pGPphoAdns in dns of C6709, Smr, Apr | This study |
| ΔhapRdns::pGPphoA | Insertion of pGPphoAdns in dns of ΔhapR, Smr, Apr | This study |
| C6709vpsA::pGPphoA | Insertion of pGPphoAvpsA in vpsA of C6709 Smr, Apr | This study |
| ΔhapRvpsA::pGPphoA | Insertion of pGPphoAvpsA in vpsA of ΔhapR, Smr, Apr | This study |
| C6709xds::pGPphoA | Insertion of pGPphoAxds in xds of C6709, Smr, Apr | This study |
| ΔhapRxds::pGPphoA | Insertion of pGPphoAxds in xds of ΔhapR, Smr, Apr | This study |
| Plasmids | ||
| pCVD442 | oriR6K mobRP4 sacB, Apr | Donnenberg and Kaper (1991) |
| pGPphoA | pGP704 with promotorless phoA of SM10λpir, Apr | Moisi et al. (2009) |
| pMMB | pMMB67EH, IncQ broad-host-range low-copy-number cloning vector, IPTG inducible, Apr | Morales et al. (1991) |
| pBAD24 | araBADp cloning vector, Apr | Guzman et al. (1995) |
| pCVD442ΔampD | pCVD442::ΔampD, Apr | This study |
| pCVD442Δdns | pCVD442::Δdns, Apr | This study |
| pCVD442ΔhapR | pCVD442::ΔhapR, Apr | This study |
| pCVD442ΔvpsA | pCVD442::ΔvpsA, Apr | This study |
| pCVD442Δxds | pCVD442::Δxds, Apr | This study |
| pGPphoAdns | pGPphoA with ‘dns’ fragment of C6709, Apr | This study |
| pGPphoAvpsA | pGPphoA with ‘vpsA’ fragment of C6709, Apr | This study |
| pGPphoAxds | pGPphoA with ‘xds’ fragment of C6709, Apr | This study |
| pampD | ampD of C6709 in pMMB, Apr | This study |
| pdns | dns of C6709 in pMMB, Apr | This study |
| pxds | xds of C6709 in pMMB, Apr | This study |