TABLE 1.
PCR primers for detection of human intestinal bifidobacteria
| Target | Primer | Sequence | Product size (bp) |
|---|---|---|---|
| Bifidobacterium | g-Bifid-F | CTCCTGGAAACGGGTGG | 549-563 |
| g-Bifid-R | GGTGTTCTTCCCGATATCTACA | ||
| B. adolescentis groupa | BiADOg-1a | CTCCAGTTGGATGCATGTC | 279 |
| BiADOg-1b | TCCAGTTGACCGCATGGT | ||
| BiADO-2 | CGAAGGCTTGCTCCCAGT | ||
| B. angulatum | BiANG-1 | CAGTCCATCGCATGGTGGT | 275 |
| BiANG-2 | GAAGGCTTGCTCCCCAAC | ||
| B. bifidum | BiBIF-1 | CCACATGATCGCATGTGATTG | 278 |
| BiBIF-2 | CCGAAGGCTTGCTCCCAAA | ||
| B. breve | BiBRE-1 | CCGGATGCTCCATCACAC | 288 |
| BiBRE-2 | ACAAAGTGCCTTGCTCCCT | ||
| B. catenulatum group | BiCATg-1 | CGGATGCTCCGACTCCT | 285 |
| BiCATg-2 | CGAAGGCTTGCTCCCGAT | ||
| B. longumb | BiLON-1 | TTCCAGTTGATCGCATGGTC | 831 |
| BiLON-2 | GGGAAGCCGTATCTCTACGA | ||
| B. infantisb | BiINF-1 | TTCCAGTTGATCGCATGGTC | 828 |
| BiINF-2 | GGAAACCCCATCTCTGGGAT | ||
| B. dentium | BiDEN-1 | ATCCCGGGGGTTCGCCT | 387 |
| BiDEN-2 | GAAGGGCTTGCTCCCGA |
a
The B. adolescentis group consisted of B. adolescentis genotypes A and B. BiADOg-1a, BiADOg-1b, and BiADO-2 are used in the same PCR mixture. BiADOg-1a is the primer used to detect B. adolescentis genotype A and is synonymous with BiADO-1 (18). BiADOg-1b is the primer used to detect B. adolescentis genotype B.
b
B. longum, B. longum biotype longum; B. infantis, B. longum biotype infantis (27).