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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Physiol. 2012 Jun;227(6):2341–2351. doi: 10.1002/jcp.22969

Fig. 1. Overexpression of the N-ras proto-oncogene reduces the oncogenic transformation in SV40 transformed cells.

Fig. 1

A, NIH3T3 SV40 transformed cells (SV5) and cells expressing both SV40T and wt N-ras (SV5-NN clones) were immunobloted with antibodies against N-ras and SV40. As positive control, NIH3T3 cells transformed with the N-ras oncogene (NrasT) were used. B, Introduction of the N-ras proto-oncogene in SV40 transformed cells significantly reduces anchorage independence). * p<0.05. C, N-ras proto-oncogene (wt N-ras) reduces proliferation in SV40 transformed cells. SV5 and SV5-NN cells (clones 2 and 4) were seeded on day 0 and counted every three days over two weeks while growing in 10% or 1% serum. D, Indirect determination of cell proliferation rate by colorimetric assessment of MTT to formazan conversion. SV5 and SV5-NN4 cells were plated at 1 × 104 cells per well of 96-well plate, and optical density (OD) was measured at 550 nm using a plate reader. Data are expressed as means ± SD (n = 3). * p<0.05.