Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Physiol. 2012 Jun;227(6):2341–2351. doi: 10.1002/jcp.22969

Fig. 3. N-ras proto-oncogene decreases oncogenic transformation in human tumor cell lines.

Fig. 3

A, Introduction of the N-ras proto-oncogene in HT1080 and MDA-MB-231 cells significantly reduces its migration ability. Both cell lines infected with pMIGR1 (GFP) or pMIGR1-wt N-ras (GFP-wt N-ras) were seeded and the pictures were taken at time 0 and 24 hours after the wound was made. B, Quantification of migration by measuring the distance between the invading fronts of cells in three random selected microscopic fields for each condition. The degree of migration is expressed as percent of uncovered wound. C, N-ras proto-oncogene reduces human tumor cell growth on a soft agar assay. HT1080 and MDA-MB-231 cells non infected (Control) or infected with pMIGR1 and pMIGR1-wt N-ras (GFP-wtNras) were seeded on day 0 and the number of colonies was counted two weeks later. Data represent mean of three independent experiments. * p<0.05.