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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Physiol. 2012 Jun;227(6):2341–2351. doi: 10.1002/jcp.22969

Fig 5. Reconstitution of the TGFβ pathway restores migration but not anchorage independence.

Fig 5

A, SV5-NN4 clones stably expressing a constitutively activated TGFβ receptor were transiently transfected with 3TPLux, a TGFβ-inducible reporter plasmid. 24 hours after transfection, cells were treated or not with TGFβ during 24 hours. At 48 h post-transfection cells were lysed and firefly luciferase activity was determined. Bars represent the mean ± S.D. of 3 independent experiments. B, Wound healing of SV5-NN4 cells and SV5-NN4 cells expressing stably the constitutively activated TGFβ receptor I at times 0 and 40 hours. C, Percentage of uncovered wound of three separate experiments, measured at 24 hours along the scratch wound. * p<0.05. D, Soft agar assay of SV5, SV5-NN4 and some of the TGFβ expressing clones. Cells were seeded at day cero on a semi-solid medium and counted after 14 days. E, Quantitative PCR of genes downregulated in SV5-NN4 cells reactivated by TGFβ signaling. Fold change values are normalized to NN4 cells using Msgt3 as housekeeping gene.