TABLE 1.

Relative activities of WT and mutant DAOCSs determined via bioassay

DAOCS enzyme	Relative activity (%)a
	Penicillin G	Ampicillin	Amoxicillin	Carbenicillin	Phenethicillin	Metampicillin	Penicillin V
WT	100	100	100	100	100	100	100
N304L	129	163	226	235	173	153	415
N304A	163	192	221	242	163	216	333
N304G	42	43	67	37	65	57	60
N304I	29	63	NDb	42	58	84	89
N304V	29	43	ND	ND	64	42	89
N304P	ND	ND	ND	ND	ND	ND	ND
N304S	80	62	48	68	80	74	80
N304C	44	24	ND	20	27	43	48
N304T	38	35	35	21	54	54	76
N304M	92	120	139	161	127	137	237
N304F	19	11	ND	ND	23	68	ND
N304Y	22	13	ND	ND	24	39	ND
N304W	20	16	ND	15	15	43	ND
N304D	18	12	ND	15	10	38	ND
N304E	44	24	ND	13	34	48	ND
N304K	242	158	521	404	222	371	733
N304R	263	346	708	280	360	576	493
N304H	60	39	46	28	50	60	36
N304Q	44	76	75	54	67	95	103

^aThe relative activity of each mutant enzyme is expressed as the percentage of the specific activity of the mutant enzyme relative to that of the WT enzyme, which was set at 100%.

^bND, not detectable.