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. 1999 Feb;119(2):507–510. doi: 10.1104/pp.119.2.507

Table I.

Identification of metabolites after feeding ent-kaurene to yeast transformed with the Arabidopsis GA3 clone

Putativeaand Reference Compoundb KRI
Characteristic Ions
BPX-5 HP-1 Relative Abundance of Base Peakc
%
360 (M+) 345 270 257 255 241 227 187 175 161
ent-Kaurenol TMS 2244 2330 25 7 100 80 21 11 22 10 19 23
ent-Kaurenol TMS std. 2244 2329 23 7 100 72 24 14 14 11 23 26
286 (M+) 271 257 243 225 215 199 187 161
ent-Kaurenal 2231 2257 100 11 35 61 27 19 34 41 42
ent-Kaurenal std. 2233 2258 100 9 35 62 27 12 35 36 34
316 (M+) 301 273 257 256 241 213 199 187 159
ent-Kaurenoic acid methyl ester 2242 2286 100 29 55 92 38 69 29 18 27 22
ent-Kaurenoic acid methyl ester std. 2242 2285 100 26 52 93 37 69 29 19 19 21
a

Samples were analyzed, following appropriate derivatization, by full-scan GC-MS on both BPX-5 and HP-1 capillary columns. 

b

Comparison was with unlabeled standards (std.), and all samples were coinjected with parafilm so that KRI values could be compared. 

c

Data are from full scan on an HP-1 column.