Table I.
Identification of metabolites after feeding ent-kaurene to yeast transformed with the Arabidopsis GA3 clone
Putativeaand Reference Compoundb | KRI
|
Characteristic
Ions
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
BPX-5 | HP-1 | Relative Abundance of Base Peakc | ||||||||||
% | ||||||||||||
360 (M+) | 345 | 270 | 257 | 255 | 241 | 227 | 187 | 175 | 161 | |||
ent-Kaurenol TMS | 2244 | 2330 | 25 | 7 | 100 | 80 | 21 | 11 | 22 | 10 | 19 | 23 |
ent-Kaurenol TMS std. | 2244 | 2329 | 23 | 7 | 100 | 72 | 24 | 14 | 14 | 11 | 23 | 26 |
286 (M+) | 271 | 257 | 243 | 225 | 215 | 199 | 187 | 161 | ||||
ent-Kaurenal | 2231 | 2257 | 100 | 11 | 35 | 61 | 27 | 19 | 34 | 41 | 42 | |
ent-Kaurenal std. | 2233 | 2258 | 100 | 9 | 35 | 62 | 27 | 12 | 35 | 36 | 34 | |
316 (M+) | 301 | 273 | 257 | 256 | 241 | 213 | 199 | 187 | 159 | |||
ent-Kaurenoic acid methyl ester | 2242 | 2286 | 100 | 29 | 55 | 92 | 38 | 69 | 29 | 18 | 27 | 22 |
ent-Kaurenoic acid methyl ester std. | 2242 | 2285 | 100 | 26 | 52 | 93 | 37 | 69 | 29 | 19 | 19 | 21 |
Samples were analyzed, following appropriate derivatization, by full-scan GC-MS on both BPX-5 and HP-1 capillary columns.
Comparison was with unlabeled standards (std.), and all samples were coinjected with parafilm so that KRI values could be compared.
Data are from full scan on an HP-1 column.