Table II.
Summary of intermediates detected after a 1-h incubation of yeast expressing the GA3 cDNA with ent-kaurene, ent-kaurenol, and ent-kaurenal
Feed | Intermediates Detected by GC-MS
|
|||
---|---|---|---|---|
ent-Kaurene | ent-Kaurenol | ent-Kaurenal | ent-Kaurenoic acid | |
ent-Kaurene | Yes | Yes | Yes | Yes |
ent-Kaurenol | n.d.a | Yes | n.d. | Yes |
ent-Kaurenal | n.d. | n.d. | n.d. | Yes |
n.d., Not detected.