TABLE 2.
Gene | Forward primer | Reverse primer | Annealing temp (°C) |
---|---|---|---|
adk | 5′CAGCTATGAAGGCTGAAACTG3′ | 5′CTAAGCCTCCGATGAGAACA3′ | 57 |
ccpA | 5′GTTTAGGATACCGCCCAAATG3′ | 5′TGTAACTTCTTCGCGCTTCC3′ | 59 |
ftsA | 5′TCTTGACATCGGTACATCCA3′ | 5′GCCTGTAATAAGTGTACCTTCCA3′ | 57 |
glpT | 5′TGCGGCTGGATGAGTGA3′ | 5′AAGTAAGAGCAAGGAAGA3′ | 52 |
pyrE | 5′TCGCATCGCATTTATTAGAA3′ | 5′CCTGCTTCAAGCTCGTATG3′ | 57 |
recF | 5′GCGATGGCGAAATCTCATAG3′ | 5′CAAATCCATTGATTCTGATACATC3′ | 59 |
sucC | 5′GGCGGAACAGAAATTGAAGA3′ | 5′TCACACTTCATAATGCCACCA3′ | 59 |