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. 2004 Jan;70(1):191–201. doi: 10.1128/AEM.70.1.191-201.2004

TABLE 2.

Primers used for PCR amplification and sequencing of B. cereus group housekeeping genes

Gene Forward primer Reverse primer Annealing temp (°C)
adk 5′CAGCTATGAAGGCTGAAACTG3′ 5′CTAAGCCTCCGATGAGAACA3′ 57
ccpA 5′GTTTAGGATACCGCCCAAATG3′ 5′TGTAACTTCTTCGCGCTTCC3′ 59
ftsA 5′TCTTGACATCGGTACATCCA3′ 5′GCCTGTAATAAGTGTACCTTCCA3′ 57
glpT 5′TGCGGCTGGATGAGTGA3′ 5′AAGTAAGAGCAAGGAAGA3′ 52
pyrE 5′TCGCATCGCATTTATTAGAA3′ 5′CCTGCTTCAAGCTCGTATG3′ 57
recF 5′GCGATGGCGAAATCTCATAG3′ 5′CAAATCCATTGATTCTGATACATC3′ 59
sucC 5′GGCGGAACAGAAATTGAAGA3′ 5′TCACACTTCATAATGCCACCA3′ 59