Table I.
Cell numbers in bone marrow and spleen of normal and mutant mice
| Fraction |
||||||||
|---|---|---|---|---|---|---|---|---|
| Bone marrow | N ≥ | Total cells x 106a | CD19+ x 106 | B x 105 | C x 105 | D x 105 | E x 105 | F x 105 |
| WT | 9 | 12.9 (0.3) | 3.0 (0.2) | 2.6 (0.3) | 2.1 (0.3) | 19.0 (1.4) | 7.1 (0.4) | 3.9 (0.5) |
| ΔD-iD | 12 | 11.6 (0.4)b | 2.1 (0.2)d | 4.3 (0.6)b | 2.1 (0.3) | 13.6 (1.2)c | 4.9 (0.5)b | 1.9 (0.3)d |
| Spleen | Total cells x 106 | CD19 + x 106 | T1 x 105 | T2 x 105 | T3 x 105 | MZ x 105 | M x 105 | |
| WT | 8 | 53.9 (3.3) | 22.5 (1.0) | 1.1 (0.1) | 1.3 (0.1) | 2.2 (0.1) | 1.4 (0.1) | 14.5 (1.4) |
| ΔD-iD | 10 | 42.3 (1.7)d | 14.3 (0.6)e | 1.2 (0.1) | 1.2 (0.0) | 1.2 (0.1)e | 1.9 (0.2)b | 7.8 (0.5)e |
| Peritoneal cavity | Total cells x 105 | CD19 + x 105 | B1a x 105 | B1b x 105 | B2 x 105 | |||
| WT | 10 | 31.0 (3.2) | 11.5 (0.7) | 4.2 (0.4) | 1.4 (0.2) | 2.4 (0.3) | ||
| ΔD-iD | 10 | 23.7 (1.4)d | 7.8 (0.6)e | 1.9 (0.6)e | 1.0 (0.1) | 2.8 (0.3) | ||
Total nucleated cells that excluded trypan blue. In the bone marrow, values shown are cell counts per femur (average cellularity of two femurs collected from each experimental animal) of paired 8-wk-old homozygous ΔD-iD (ΔD-iD) or homozygous wild-type (WT) littermate progeny of heterozygous ΔD-iD/WT BALB/c mice. The standard error of the mean is shown in parentheses. The number of cells in fractions B (CD19+CD43+HSA+BP-1−), C (CD19+CD43+HSA+BP-1+), D (CD19+CD43−IgM−IgD−), E (CD19+CD43−IgM+IgD−), and F (CD19+CD43−IgMloIgDhi) was determined from the relative proportion of total cells. Cell counts per spleen of 8-wk-old homozygous ΔD-iD or wt littermate progeny of WT BALB/c mice. In the spleen, transitional T1 (CD19+AA4.1+sIgMhiCD23−), T2 (CD19+AA4.1+sIgMhiCD23+), and T3 (CD19+AA4.1+sIgMintCD23+) splenic B cell subsets were determined as described by Allman et al. (reference 46). Marginal zone (MZ, CD19+CD21hiCD23lo) and mature (M, CD19+CD21loCD23hi) B cell subsets were determined as described by Oliver et al. (reference 47). In the peritoneal cavity, values shown are average cell counts per single peritoneal lavage of 8-wk-old homozygous ΔD-iD or WT littermates. The number of B1a (CD19+CD5+), B1b (CD19+Mac-1+CD5−), and B2 (CD19+Mac-1−CD5−) cells were determined from the relative proportion of total cells.
P ≤ 0.05.
P ≤ 0.01.
P ≤ 0.005.
P ≤ 0.001 versus BALB/c wild-type littermates.