FIG. 1.

Alignment of laccase sequences from M. albomyces (MaL), P. anserina (PaL; accession number P78722) (14), and N. crassa (NcL; accession number P06811) (17) as determined with the Clustal W multiple-sequence alignment program (42). An asterisk indicates that the residues at a position are identical in all sequences in the alignment, a colon indicates that conserved substitutions have been observed, and a period indicates semiconserved substitutions. Putative signal sequences are indicated by italics, the propeptides are underlined, and the conserved C-terminal cleavage site is indicated by an arrow. The conserved residues involved in copper binding are enclosed in boxes, and the peptide sequences obtained from purified M. albomyces laccase (26) are overlined. The possible N-glycosylation sites are indicated by boldface type, and the shaded amino acids indicate the locations of introns in the DNA sequence.