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Western blot analysis of culture supernatants (lanes 1, 3, 5, and 7) and cell lysates (lanes 2, 4, 6, and 8) of S. cerevisiae transformants after 3 days of induction with galactose. S. cerevisiae was transformed with pYES2 (lanes 1 and 2), pLLK10 (lanes 3 and 4), pMS174 (lanes 5 and 6), and pMS175 (lanes 7 and 8). Fifteen microliters of the supernatant and a corresponding amount of cell lysate were loaded on the gel. Lane 9 contained 30 ng of native M. albomyces laccase.
